Dysfunction or absence of the trace amine associated receptor-1 (TAAR-1) has been shown to increase methamphetamine (METH) consumption in mice. Using C57BL/6J mice (functional TAAR-1 and exhibit low levels of METH consumption) crossed with DBA/2J mice (lack a functional TAAR-1 and exhibit high levels of METH consumption) our collaborators created METH low drinking (MALDR) and METH high drinking (MAHDR) mice. The objective of the proposed study is to determine the circadian phenotype of mice with a non-functional TAAR-1 (MAHDR) compared to mice with a functional TAAR-1 (MALDR). We hypothesize that the MAHDR mice will show a distinct circadian phenotype compared to the MALDR mice. The project will assess circadian and behavioral differences between the MALDR and MAHDR genotypes on: a) basal activity-like, ingestive-like, exploratory/anxiety-like, and sleep-like behaviors using the video behavioral analysis system in 12:12 light-dark (LD) cycle; b) re-entrainment rate of onset of circadian wheel running activity to a 6h phase advance of the dark onset; c) anticipatory activity in response to saline or METH injections; d) circadian profile in a constant dark (DD) environment to assess differences in endogenous rhythms; e) phase shift in response to a light pulse in DD environment; f) differences in desynchronization rate of subjecting mice to a forced desynchrony paradigm (11:11 LD cycle); and g) effectiveness of melatonin treatment in reconsolidation of desynchronized running wheel activity pattern. Mice may also be tested for anxiety-like behavior via the open field test and marble burying test.
Disciplines: Pharmacology and toxicology, neuroscience
Student Skill-Set Needed: Ability to work independently, open and flexible schedule, motivation, comfortable working with animals
Compensation: Academic Credit, Volunteer, Work Study
Available: Fall, Summer
For further information on this opportunity, or to apply, contact:
Faculty Member: Margarita L. Dubocovich, Phd.
Title: SUNY Distinguished Professor
Department: Pharmacology And Toxicology
Office: 101A Farber Hall