Student working on a research project.

The Effects Of Protein Arginine Methylation On Pre-mRNA Splicing

During gene expression, pre-mRNAs are processed by a multitude of RNA processing factors, including components of the spliceosomal complex, and packaged into messenger ribonucleoparticles (mRNPs) before being exported to the cytoplasm as mature, translatable mRNAs. Since mRNP biogenesis involves an intricate web of cross-stimulatory connections and physical interdependencies, the formation of an export-competent mRNP requires that such processing factors be recruited onto nascent transcripts co-transcriptionally, and in a very precise order. In yeast, ordered association of the spliceosomal components during transcription facilitates efficient co-transcriptional mRNA processing, but the mechanisms regulating these ordered associations remain largely unknown. Previous studies from our laboratory, using yeast Saccharomyces cerevisiae, demonstrated that the major protein arginine methyltransferase (PRMT) Hmt1 plays an important role in proper co-transcriptional recruitment of a number of mRNA processing factors. We identified Snp1, a U1 snRNP component, as a substrate of Hmt1. Cells lacking Hmt1 or its catalytic activity display changes in the co-transcriptional recruitment to the genomic targets for a number of splicing factors. Our lab is currently trying to understand the molecular mechanism by which Hmt1 modulates the recruitment as well as other aspects of pre-mRNA splicing.

Research Project Information

Student Skill-Set Needed: fundamental knowledge of molecular biology
Compensation: Academic Credit, Volunteer, Work Study
Available: Fall, Spring, Summer
Website: http://www.biology.buffalo.edu/Faculty/Yu/yu.html

Contact

For further information on this opportunity, or to apply, contact:

Faculty Member: Michael Yu
Title: Associate Professor
Department: Biological Sciences
Office: 355 Cooke Hall
Phone: 7166454931
Email: mcyu@buffalo.edu