Student working on a research project.

XPO7 Recombinant Protein Expression And Detection

The student will work on the cloning of mouse XPO7 cDNA into a prokaryotic expression vector to allow XPO7 to be expressed in E. coli. Upon successful cloning of the cDNA, the student will document correct expression of the protein and apply western blot experiments to determine the level of reactivity and specificity of a polyclonal antibody that was previously generated against an XPO7 synthetic peptide. Techniques to be applied are RNA isolation, primer design, RT-PCR, ligation, bacterial transformation, plasmid isolation, interpretation of DNA sequencing, bacterial cell culture and western blot analyses.

Research Project Information

Disciplines: Hematology, molecular biology
Student Skill-Set Needed: Biotechnology or Medical Technology major in good academic standing who has at least taken a course in biochemistry
Compensation: Academic Credit
Available: Fall, Spring
Website: http://medicine.buffalo.edu/content/medicine/faculty/profile.html?ubit=stvkoury

Contact

For further information on this opportunity, or to apply, contact:

Faculty Member: Stephen Koury
Title: Assistant Professor
Department: Biotechnical And Clinical Laboratory Sciences
Office: 26 Cary Hall
Phone: 829-5188
Email: stvkoury@buffalo.edu